Pengasingan dan Pengesanan Asid Deoksiribonukleik (DNA) Spesies Haiwan menggunakan Multipleks Tindak Balas Rantaian Polimerase (PCR) daripada Minyak Sapi
نویسندگان
چکیده
Kajian ini dijalankan untuk mengesan asid deoksiribonukleik (DNA) spesies haiwan dalam campuran minyak sapi menggunakan teknik tindak balas rantaian polimerase (PCR) multipleks. Kebolehdapatan DNA daripada produk berminyak membolehkan analisis PCR dan sekaligus boleh menentukan kehadiran tersebut dengan primer yang khusus-spesies. Gen sasaran adalah mitokondria (mtDNA) Sitokrom oksidase II (COII) lembu, manakala mtDNA tRNA-ATP8 nuklear (nDNA) Elemen Nuklear Berselang pendek (SINE) babi. Terdapat satu kawalan (S1) tiga sampel gelatin babi ditambah pada kepekatan berbeza iaitu 1%, 3% 5% (w/v) (S2, S3 S4). Analisis simpleks spesies-spesifik disebutkan, berjaya mengamplifikasi lembu amplikon bersaiz 165 bp 212 (tRNA-ATP8) 161 (SINE). Manakala, multipleks telah dioptimumkan nDNA babi, juga menghasilkan sama. Oleh itu, kajian menunjukkan bahawa kaedah mudah, ringkas pantas di selain simpleks.
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ژورنال
عنوان ژورنال: Sains Malaysiana
سال: 2021
ISSN: ['0126-6039', '2735-0118']
DOI: https://doi.org/10.17576/jsm-2021-5011-11